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KINETICS OF SULFITE OXIDASE PURIFIED FROM MALVA SYLVESTRIS

Abstract

Sulfite oxidase was purified from Malva sylvestris (Sunchul) leaves by acetone fractionation, heat treatment , ion - exchange chromatography and gel permeation chromatography methods. The activity of the enzyme was determined using a coupled assay with sodium sulfite as substrate and potassium ferricyanide as co - substrate. Decrease in absorbance at 420nm was monitored.The pH and temperature optima of the enzyme were found to be 7.8 and 30°C , respectively. Km and Vmax as determined by using different methods were 3.34mM and 1.13mM/min respectively. The activation energy of the enzyme was 71.3kj/mole.

B.A. GANAI, A. MASOOD, M.A. ZARGAR and M.B. SYED

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