Research Article Open Access

PRODUCTION AND PARTIAL PURIFICATION OF PEROXIDASE FROM WATER HYACINTH PLANTS INDUCED BY TEXTILE DYEING EFFLUENT

Abstract

Water hyacinth is the “Cinderella” of the plant world. It has the ability to treat industrial effluents. In the present study, production and partial purification of peroxidase from acclimatized water hyacinth roots has been studied. Initially, the water hyacinth plants were gradually acclimatized to textile dyeing effluent from 5% to 50%. The production of enzyme was greatly induced by the strength of the effluent. The enzyme was purified by fractional precipitation using ammonium sulphate, dialysis and gel exclusion chromatography. The specific activity of peroxidase in crude extract was 6.25 x 10-2 and it was significantly increased to 2 in the purified enzyme sample. The results indicated that, acclimatized water hyacinth roots could be a simple and easily available source for cost effective industrial production of peroxidase.

R. MALA AND S. SARAVANA BABU

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